I did the amylase inhibition assay and confirm the color of final mixture was good. Enzymatic method for determining amylase activity amylase. Ghi on pancreatic amylase was observed over a wide range of. After measuring absorbance, inhibition % was calculated. The absorbance of the mixture measured at 517 nm after 2 min. Amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric 405 nm product, proportional to the amount of substrate, ethylidenepnpg7. This assay protocol is suitable for the colorimetric detection of amylase activity in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the amylase activity assay kit mak009.
Without seeing your data, it is somewhat for me to say but you should read this book, which can be downloaded freely from the internet. L of the test extract was allowed to react with 200. The latter is a fairly new method while the former is a traditional method of enzyme production from microbes which has been in use for a longer period of time. Alphaamylase inhibition and antioxidant activity of marine green. Amylase inhibitory activities of selected medicinal plants. The inhibition assay was performed using the chromogenic dnsa. Alpha amylase inhibitory activitv of some plant extracts with. To evaluate the alpha amylase inhibitory activity of different extract. Alphaamylase inhibition and antioxidative capacity of some. We found that the free flavonoid extract had higher 43. Antioxidant activity was evaluated in terms of free radical scavenging, reducing power. Briefly, 250 l of the 5 mgml extract was preincubated with 250 l of. The samples were incubated in dark for 30 min, and absorbance was measured at.
The free radical scavenging activity was found to be prominent against. The alphaamylase inhibition assay was done using the chromogenic. The extracts were then assayed for alphaamylase inhibitory activity. Antioxidant activity was evaluated in terms of free radical scavenging, reducing.
It is also the major enzyme produced during the malting process. In the previous chapter some the extracts shown free radical scavenging property. The enzyme is very stable and can be incubated at 60c during the mashing process in the brewery. The objective of the present study was to provide an invitro evidence for the potential inhibitory activity of extracts and fractions of adiantum caudatum linn.
All the plant extracts exhibited a relative alphaamylase inhibition. The alphaamylase inhibition assay was done using the. Alpha amylase inhibition assay principle and procedure. Chapter4 antidiabetic activity of the selected plants by. In vitro amylase inhibition was studied by the method of bernfeld. All the plant extracts exhibited a relative alphaamylase inhibition apart from. Chapter4 antidiabetic activity of the selected plants by in vitro models the selected 15 medicinal plants.
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